Ribosome stalling is responsible for arginine-specific translational attenuation in Neurospora crassa. Academic Article

abstract

• The Neurospora crassa arg-2 upstream open reading frame (uORF) plays a role in negative arginine-specific translational regulation. Primer extension inhibition analyses of arg-2 uORF-containing RNA translated in a cell-free system in which arginine-specific regulation was retained revealed "toeprints" corresponding to ribosomes positioned at the uORF initiation and termination codons and at the downstream initiation codon. At high arginine concentrations, the toeprint signal corresponding to ribosomes at the uORF termination codon rapidly increased; a new, broad toeprint that represents additional ribosomes stalled on the uORF appeared 21 to 30 nucleotides upstream of this site; and the toeprint signal corresponding to ribosomes at the downstream initiation codon decreased. These data suggest that arginine increases ribosomal stalling and thereby decreases translation from the downstream initiation codon.

authors

• Sachs, Matthew

published proceedings

• Mol Cell Biol

author list (cited authors)

• Wang, Z., & Sachs, M. S.

citation count

• 108

complete list of authors

• Wang, Z||Sachs, MS

publication date

• September 1997

publisher

• Taylor & Francis  Publisher

keywords

• Amino Acid Sequence
• Arginine
• Base Sequence
• Carbon-nitrogen Ligases With Glutamine As Amide-n-donor
• Cycloheximide
• Genes, Reporter
• Ligases
• Luciferases
• Molecular Sequence Data
• Mutation
• Neurospora Crassa
• Open Reading Frames
• Protein Biosynthesis
• Protein Synthesis Inhibitors
• Puromycin
• RNA
• Ribosomes

PubMed Central ID

• 9271370

Digital Object Identifier (DOI)

• 10.1128/MCB.17.9.4904

start page

• 4904

end page

• 4913

volume

• 17

issue

• 9

URL

• http%3A%2F%2Fdx.doi.org%2F10.1128%2Fmcb.17.9.4904