Transformation of Medicago truncatula via infiltration of seedlings or flowering plants with Agrobacterium Academic Article uri icon


  • Two rapid and simple in planta transformation methods have been developed for the model legume Medicago truncatula. The first approach is based on a method developed for transformation of Arabidopsis thaliana and involves infiltration of flowering plants with a suspension of Agrobacterium. The second method involves infiltration of young seedlings with Agrobacterium. In both cases a proportion of the progeny of the infiltrated plants is transformed. The transformation frequency ranges from 4.7 to 76% for the flower infiltration method, and from 2.9 to 27.6% for the seedling infiltration method. Both procedures resulted in a mixture of independent transformants and sibling transformants. The transformants were genetically stable, and analysis of the T2 generation indicates that the transgenes are inherited in a Mendelian fashion. These transformation systems will increase the utility of M. truncatula as a model system and enable large-scale insertional mutagenesis. T-DNA tagging and the many adaptations of this approach provide a wide range of opportunities for the analysis of the unique aspects of legumes.

altmetric score

  • 6

author list (cited authors)

  • Trieu, A. T., Burleigh, S. H., Kardailsky, I. V., Maldonado-Mendoza, I. E., Versaw, W. K., Blaylock, L. A., ... Harrison, M. J.

citation count

  • 195

complete list of authors

  • Trieu, AT||Burleigh, SH||Kardailsky, IV||Maldonado-Mendoza, IE||Versaw, WK||Blaylock, LA||Shin, H||Chiou, TJ||Katagi, H||Dewbre, GR||Weigel, D||Harrison, MJ

publication date

  • June 2000