An Improved Colorimetric Assay for Bacterial Lipase in Nonfat Dry Milk Academic Article

abstract

• ABSTRACTA modification of McKellar and Cholette's (1986) colorimetric method for determining lipase activity in reconstituted nonfat dry milk was developed. The method uses the color reaction between Fast Blue BB and the naphthol (BN) enzymatically cleaved from naphthyl caprylate (BNC) as a measure of lipase activity. In the modified method, a solvent system is used to clarify the sample rather than extracting the colored product. This modification allows measurement of total sample lipase by excluding centrifugation. In addition, the modified method has greater sensitivity with equal ease of use.

authors

• Versaw, Wayne

published proceedings

• Journal of Food Science

author list (cited authors)

• VERSAW, W. K., CUPPETT, S. L., WINTERS, D. D., & WILLIAMS, L. E.

complete list of authors

• VERSAW, WK||CUPPETT, SL||WINTERS, DD||WILLIAMS, LE

publisher

• Wiley  Publisher

published in

• JOURNAL OF FOOD SCIENCE  Journal

keywords

• 30 Agricultural, Veterinary And Food Sciences
• 3006 Food Sciences

Digital Object Identifier (DOI)

• 10.1111/j.1365-2621.1989.tb05159.x

start page

• 1557

end page

• 1558

volume

• 54

issue

• 6