OPTIMIZATION OF LISTERIOLYSIN-O PRODUCTION BY LISTERIA-MONOCYTOGENES

abstract

• Listeria monocytogenes serotype 1 and Scott A strains were examined to determine the effect of culture conditions onproduction of listeriolysin O (LLO). The hemolytic activity was assayed by hemolysis of sheep red blood cells. The optimum pH range for LLO production in serotype 1 and Scott A were pH 6.26.0 and pH 5.86.0, respectively. Production of LLO by serotype 1 was 20fold higher than by Scott A. Addition of more than 1.0% glucose significantly reduced production of LLO by the cells, while favoring growth. By adding 0.5% glucose after stationary phase and adjusting pH to 5.57.0, maximum LLO was produced after 2 h incubation at 37C by both strains. Copyright 1994, Wiley Blackwell. All rights reserved

authors

• Murano, Elsa

published proceedings

• JOURNAL OF FOOD SAFETY

author list (cited authors)

• KIM, K., MURANO, E. A., & OLSON, D. G.

citation count

• 0

complete list of authors

• KIM, K||MURANO, EA||OLSON, DG

publication date

• January 1994

publisher

• Wiley  Publisher

published in

• Journal of Food Safety  Journal

keywords

• Foodborne Illness

Digital Object Identifier (DOI)

• 10.1111/j.1745-4565.1994.tb00600.x

start page

• 281

end page

• 288

volume

• 14

issue

• 4

URL

• http%3A%2F%2Fdx.doi.org%2F10.1111%2Fj.1745-4565.1994.tb00600.x