Selecting high-growth/high-lipid producing microalgae from a mutant library through droplet microfluidics Conference Paper uri icon


  • We have developed a high-throughput droplet microfluidics-based screening platform for analyzing growth and lipid production of a large number of microalgal mutants, followed by selective restoration of strains that show enhanced growth and higher lipid production. Through an on-chip lipid staining process as well as an integrated optical detection system, growth and lipid production of a single mutated cell in droplets can be quantified by measuring chlorophyll autofluorescence and BODIPY fluorescence intensities, respectively. The performance of the developed screening platform was demonstrated by examining a randomly mutated unicellular microalga Chlamydomonas reinhardtii library.

published proceedings

  • 20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016

author list (cited authors)

  • Kim, H. S., Han, S., Guzman, A. R., Sobahi, N., Thapa, H. R., Browne, D., ... Han, A.

complete list of authors

  • Kim, HS||Han, S||Guzman, AR||Sobahi, N||Thapa, HR||Browne, D||Tatli, M||Hsu, S||Stern, DB||Devarenne, TP||Han, A

publication date

  • January 2016