Chromosome-specific microsatellite multiplex sets for linkage studies in the domestic dog. Academic Article

abstract

• To expedite linkage studies and positional cloning efforts in the dog, Minimal Screening Set 2 (MSS-2) of 327 canine microsatellite markers has been multiplexed into chromosome-specific panels. MSS-2 provides 9 Mb coverage of the canine genome with no gaps larger than 17.1 Mb and is the most recent and comprehensive set of microsatellites available for whole-genome scans. Markers were labeled with fluorescent dyes based on locations and expected product sizes to facilitate the multiplexing of a maximum number of markers for each chromosome. All markers are amplified using a single thermal cycling program and PCR mix and are optimized for resolution on an ABI 3100 genetic analyzer. Sixty-nine chromosome-specific panels were created by coamplification of a maximum number of markers and subsequent coloading of the remaining markers.

authors

• Steiner, Joerg

published proceedings

• Genomics

author list (cited authors)

• Clark, L. A., Tsai, K. L., Steiner, J. M., Williams, D. A., Guerra, T., Ostrander, E. A., Galibert, F., & Murphy, K. E.

citation count

• 47

complete list of authors

• Clark, Leigh Anne||Tsai, Kate L||Steiner, Jörg M||Williams, David A||Guerra, Trina||Ostrander, Elaine A||Galibert, Francis||Murphy, Keith E

publication date

• September 2004

publisher

• Elsevier  Publisher

published in

• Genomics  Journal

keywords

• Animals
• Chromosomes, Mammalian
• Dogs
• Fluorescent Dyes
• Genetic Linkage
• Microsatellite Repeats

PubMed Central ID

• 15498461

Digital Object Identifier (DOI)

• 10.1016/j.ygeno.2004.06.006

start page

• 550

end page

• 554

volume

• 84

issue

• 3

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.ygeno.2004.06.006