Chromosome-specific microsatellite multiplex sets for linkage studies in the domestic dog. Academic Article uri icon

abstract

  • To expedite linkage studies and positional cloning efforts in the dog, Minimal Screening Set 2 (MSS-2) of 327 canine microsatellite markers has been multiplexed into chromosome-specific panels. MSS-2 provides 9 Mb coverage of the canine genome with no gaps larger than 17.1 Mb and is the most recent and comprehensive set of microsatellites available for whole-genome scans. Markers were labeled with fluorescent dyes based on locations and expected product sizes to facilitate the multiplexing of a maximum number of markers for each chromosome. All markers are amplified using a single thermal cycling program and PCR mix and are optimized for resolution on an ABI 3100 genetic analyzer. Sixty-nine chromosome-specific panels were created by coamplification of a maximum number of markers and subsequent coloading of the remaining markers.

published proceedings

  • Genomics

author list (cited authors)

  • Clark, L. A., Tsai, K. L., Steiner, J. M., Williams, D. A., Guerra, T., Ostrander, E. A., Galibert, F., & Murphy, K. E.

citation count

  • 47

complete list of authors

  • Clark, Leigh Anne||Tsai, Kate L||Steiner, Jörg M||Williams, David A||Guerra, Trina||Ostrander, Elaine A||Galibert, Francis||Murphy, Keith E

publication date

  • September 2004