Comparison of multiple chemical sanitizers for reducing Salmonella and Escherichia coli O157:H7 on spinach (Spinacia oleracea) leaves Academic Article uri icon


  • Effectiveness of multiple chemical sanitizers on the reduction of Salmonella spp. and Escherichia coli O157:H7 on spinach was compared. Fresh spinach (Spinacia oleracea) was inoculated with a bacterial suspension containing multiple strains of rifampin-resistant Salmonella and E. coli O157:H7. Inoculated spinach leaves were treated with a water wash or water wash followed by 2% L-lactic acid at 55C, peroxyacetic acid (80mg/L), calcium hypochlorite (200mg/L), ozonated water (mg/L) or ClO 2 gas (1.2 or 2.1mg/L). The l-lactic acid produced a 2.7log CFU/g reduction for E. coli O157:H7 and a 2.3log CFU/g reduction for Salmonella, statistically significant compared to water wash alone (P<0.05), which resulted in a reduction of 0.7log CFU/g for both pathogens. These findings indicate that 2% l-lactic acid at 55C may be an effective treatment for reducing pathogens on spinach leaves. 2011 Elsevier Ltd.

published proceedings


author list (cited authors)

  • Neal, J. A., Marquez-Gonzalez, M., Cabrera-Diaz, E., Lucia, L. M., O'Bryan, C. A., Crandall, P. G., Ricke, S. C., & Castillo, A.

citation count

  • 72

complete list of authors

  • Neal, Jack A||Marquez-Gonzalez, Mayra||Cabrera-Diaz, Elisa||Lucia, Lisa M||O'Bryan, Corliss A||Crandall, Philip G||Ricke, Steven C||Castillo, Alejandro

publication date

  • January 2012