Chapter Two Preparation of a Saccharomyces cerevisiae Cell-Free Extract for In Vitro Translation Academic Article uri icon


  • Eukaryotic cell-free in vitro translation systems have been in use since the 1970s. These systems can faithfully synthesize polypeptides when programmed with mRNA, enabling the production of polypeptides for analysis as well as permitting analyses of the cis- and trans-acting factors that regulate translation. Here we describe the preparation and use of cell-free translation systems from the yeast Saccharomyces cerevisiae.

author list (cited authors)

  • Wu, C., & Sachs, M. S.

publication date

  • January 1, 2014 11:11 AM