Oxytocin-induced Ca2+ responses in human myometrial cells. Academic Article uri icon


  • Complex spatiotemporal changes in intracellular Ca2+ were monitored in an immortalized human myometrial cell line (PHM1-41) and first-passage human myometrial cells after oxytocin stimulation (1. 0-1000 nM). Laser cytometry revealed intracellular Ca2+ oscillations in both culture systems starting at 1.0 nM, which were followed by repetitive Ca2+ transients by 10-15 min that lasted for at least 90 min. The amplitude of the initial Ca2+ spike was dose dependent, while the frequency of Ca2+ oscillations identified by Fast Fourier Transform (FFT) tended to increase with dose. Removal of oxytocin resulted in termination of oscillations. Analysis of the sources of the Ca2+ involved in oscillations indicated that the major contribution to oscillation frequencies of

published proceedings

  • Biol Reprod

author list (cited authors)

  • Burghardt, R. C., Barhoumi, R., Sanborn, B. M., & Andersen, J.

citation count

  • 36

complete list of authors

  • Burghardt, RC||Barhoumi, R||Sanborn, BM||Andersen, J

publication date

  • April 1999