Oxytocin-induced Ca2+ responses in human myometrial cells. Academic Article

abstract

• Complex spatiotemporal changes in intracellular Ca2+ were monitored in an immortalized human myometrial cell line (PHM1-41) and first-passage human myometrial cells after oxytocin stimulation (1. 0-1000 nM). Laser cytometry revealed intracellular Ca2+ oscillations in both culture systems starting at 1.0 nM, which were followed by repetitive Ca2+ transients by 10-15 min that lasted for at least 90 min. The amplitude of the initial Ca2+ spike was dose dependent, while the frequency of Ca2+ oscillations identified by Fast Fourier Transform (FFT) tended to increase with dose. Removal of oxytocin resulted in termination of oscillations. Analysis of the sources of the Ca2+ involved in oscillations indicated that the major contribution to oscillation frequencies of

authors

• Burghardt, Robert
• Mouneimne, Roula

published proceedings

• Biol Reprod

author list (cited authors)

• Burghardt, R. C., Barhoumi, R., Sanborn, B. M., & Andersen, J.

citation count

• 36

complete list of authors

• Burghardt, RC||Barhoumi, R||Sanborn, BM||Andersen, J

publication date

• April 1999

publisher

• Oxford University Press (OUP)  Publisher

published in

• Biology of Reproduction  Journal

keywords

• 8-Bromo Cyclic Adenosine Monophosphate
• Alprostadil
• Calcium
• Cell Line
• Dinoprostone
• Enzyme Inhibitors
• Female
• Fourier Analysis
• Humans
• Inositol 1,4,5-Trisphosphate
• Myometrium
• Oxytocin
• Periodicity
• Phosphatidylinositol Diacylglycerol-Lyase
• Pregnancy
• Type C Phospholipases

PubMed Central ID

• 10084948

Digital Object Identifier (DOI)

• 10.1095/biolreprod60.4.777

start page

• 777

end page

• 782

volume

• 60

issue

• 4

URL

• http://dx.doi.org/10.1095/biolreprod60.4.777