Use of an A-T-rich DNA clone for identification and detection of Peronosclerospora sorghi. Academic Article uri icon


  • A recombinant plasmid, pMLY12-1, screened from a Peronosclerospora sorghi library hybridizes only to DNA of P. sorghi, or to DNA from leaves infected with P. sorghi, not to DNA of P. sorghi Thailand isolate, P. philippinensis, P. sacchari, or P. maydis. The terminal sequences of the 1.3-kb insert, which appears to contain mitochondrial DNA, are 85% A and T. No polymorphisms were detected when the probe was hybridized to Southern blots containing DNA from P. sorghi pathotype 1, pathotype 3, or a Botswana isolate digested with any of the eight restriction endonucleases tested. The banding patterns were the same whether DNA was extracted directly from the fungus or from infected leaves.

published proceedings

  • Appl Environ Microbiol

author list (cited authors)

  • Yao, C. L., Magill, C. W., & Frederiksen, R. A.

citation count

  • 7

complete list of authors

  • Yao, CL||Magill, CW||Frederiksen, RA

publication date

  • July 1991