PHYLOGENETIC PLACEMENT, GENOME SIZE, AND GC CONTENT OF THE LIQUID-HYDROCARBON-PRODUCING GREEN MICROALGA BOTRYOCOCCUS BRAUNII STRAIN BERKELEY (SHOWA) (CHLOROPHYTA)1 Academic Article uri icon

abstract

  • We report the genome size and the GC content, and perform a phylogenetic analysis on Botryococcus braunii Kütz., a green, colony-forming, hydrocarbon-rich alga that is an attractive source for biopetroleum. While the chemistry of the hydrocarbons produced by the B race of B. braunii has been studied for many years, there is a deficiency of information concerning the molecular biology of this alga. In addition, there has been some discrepancy as to the phylogenetic placement of the Berkeley (or Showa) strain of the B race. To clarify its classification, we isolated the Berkeley strain nuclear SSU (18S) rRNA gene and β-actin cDNA and used these sequences for phylogenetic analysis to determine that the Berkeley strain belongs to the Trebouxiophyceae class. This finding is in agreement with other B races of B. braunii, indicating the Berkeley strain is a true B race of B. braunii. To better understand molecular aspects of B. braunii, we obtained the Berkeley strain genome size as a first step in genome sequencing. Using flow cytometry, we determined the B. braunii Berkeley genome size to be 166.2 ± 2.2 Mb. We also estimated the GC content of the Berkeley strain as 54.4 ± 1.2% for expressed gene sequences. © 2010 Phycological Society of America.

published proceedings

  • Journal of Phycology

author list (cited authors)

  • Weiss, T. L., Spencer Johnston, J., Fujisawa, K., Sumimoto, K., Okada, S., Chappell, J., & Devarenne, T. P.

citation count

  • 36

complete list of authors

  • Weiss, Taylor L||Spencer Johnston, J||Fujisawa, Kazuhiro||Sumimoto, Koremitsu||Okada, Shigeru||Chappell, Joe||Devarenne, Timothy P

publication date

  • April 2010

publisher