Surface modification of gel-free microchannel surface electrophoresis device for DNA identification Academic Article

abstract

• A gel-free microchannel electrophoresis device for DNA separation and identification was studied. DNA fragments ranging from 3.5 to 21.2kbp were effectively separated and identified. The channel's bottom surface was composed of silicon wafer or glass, and the channel wall was composed of SU-8 photoresist. The channel surface was modified with various solutions or plasmas. The separated DNA on the detection electrode was confirmed by electron spectroscopy for chemical analysis. DNA mobility on the glass substrate was higher than that on the Si substrate. In addition, the increase of the ionic strength of the solution on the device decreased the mobility of DNA. In summary, this new device is applicable to large DNA samples. 2008 The Japan Society of Applied Physics.

authors

• Kuo, Yue

published proceedings

• JAPANESE JOURNAL OF APPLIED PHYSICS

author list (cited authors)

• Lee, H. H., & Ku, Y.

citation count

• 6

complete list of authors

• Lee, Hyun Ho||Ku, Yue

publication date

• April 2008

publisher

• IOP Publishing  Publisher

published in

• Japanese Journal of Applied Physics  Journal

keywords

• Dna Electrophoresis
• Esca
• Microchannel Device
• Su-8

Digital Object Identifier (DOI)

• 10.1143/JJAP.47.2300

start page

• 2300

end page

• 2305

volume

• 47

issue

• 4

URL

• http%3A%2F%2Fdx.doi.org%2F10.1143%2Fjjap.47.2300