Cold Microwave-Enabled Protein Detection and Quantification. Academic Article

abstract

• Protein screening/detection is an essential tool in many laboratories. Owing to the relatively large time investments that are required by standard protocols, the development of methods with higher throughput while maintaining an at least comparable signal-to-noise ratio is highly beneficial in many research areas. This chapter describes how cold microwave technology can be used to enhance the rate of molecular interactions and provides protocols for dot blots, Western blots, and ELISA procedures permitting a completion of all incubation steps (blocking and antibody steps) within 24-45 min.

authors

• Steiner, Joerg

published proceedings

• Methods Mol Biol

author list (cited authors)

• Grtzner, N., Heilmann, R. M., Smith, A. G., Johnson, C. B., Vitha, S., Steiner, J. M., & Holzenburg, A. K.

citation count

• 0

complete list of authors

• Grützner, Niels||Heilmann, Romy M||Smith, Aaron G||Johnson, Carol B||Vitha, Stanislav||Steiner, Jörg M||Holzenburg, Andreas K

publication date

• January 2015

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Blotting, Western
• Electrophoresis, Polyacrylamide Gel
• Enzyme-Linked Immunosorbent Assay
• Equipment Design
• Humans
• Immunoblotting
• Microwaves
• Proteins
• Signal-To-Noise Ratio

PubMed Central ID

• 26139270

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-2718-0_23

start page

• 207

end page

• 217

volume

• 1314

URL

• http%3A%2F%2Fdx.doi.org%2F10.1007%2F978-1-4939-2718-0_23