Use of the Ussing chamber technique to study nutrient transport by epithelial tissues.

abstract

• The Ussing chamber provides a physiologically relevant system for measuring the transport of ions, nutrients, and drugs across various epithelial tissues. This article outlines the design, structure, principle, and operation of the Ussing chamber, its application in the field of gastrointestinal barrier function and nutrient transport research, as well as its advantages and limitations. This review serves as a practical guide for investigators who are new to the Ussing chamber and should help researchers better understand this valuable method for measuring the transport of electrolytes, organic nutrients, water, and drugs across the small intestine, placenta, and other epithelial tissues.

authors

• Wu, Guoyao

published proceedings

• Front Biosci (Landmark Ed)

author list (cited authors)

• He, L., Yin, Y., Li, T., Huang, R., Xie, M., Wu, Z., & Wu, G.

citation count

• 16

complete list of authors

• He, Liuqin||Yin, Yulong||Li, Tiejun||Huang, Rulin||Xie, Mingyong||Wu, Zhenlong||Wu, Guoyao

publication date

• June 2013

publisher

• IMR Press  Publisher

published in

• Frontiers in Bioscience - Landmark  Journal

keywords

• Animals
• Biological Transport
• Epithelium
• Equipment And Supplies
• Food
• Intestinal Mucosa

PubMed Central ID

• 23747882

Digital Object Identifier (DOI)

• 10.2741/4178

start page

• 1266

end page

• 1274

volume

• 18

issue

• 4

URL

• http%3A%2F%2Fdx.doi.org%2F10.2741%2F4178