Insulin action on rates of muscle protein synthesis following eccentric, muscle-damaging contractions. Academic Article uri icon

abstract

  • The purpose of this study was to determine whether eccentric, muscle-damaging contractions affect insulin action on muscle protein synthesis. Male Wistar rats (n = 28) were anaesthetized either once or twice separated by 7 days' rest, and one limb was electrically stimulated to contract eccentrically, while the contralateral limb served as a non-stimulated control. Twenty-four and 48 h after contractions, rates of protein synthesis were assessed in soleus and red or white gastrocnemius muscles during a hindlimb perfusion with or without insulin (20 000 microU mL(-1)). Rates of protein synthesis were not different in non-stimulated muscle, with or without insulin (P > 0.05). In red or white gastrocnemius without insulin, rates of protein synthesis were significantly reduced (P < 0.05) 24 and 48 h after a single session and 48 h after a double session of muscle contractions. However, protein synthesis was normalized with insulin 24 and 48 h after contractions in red, and 48 h after contractions in white gastrocnemius. In soleus muscle, protein synthesis was impaired only 48 h after the second session, but partially restored by insulin (P < 0.05). These results indicate that muscle becomes more sensitive to insulin action on rates of protein synthesis after muscle-damaging contractions.

published proceedings

  • Acta Physiol Scand

author list (cited authors)

  • Fluckey, J. D., Asp, S., Enevoldsen, L. H., & Galbo, H.

citation count

  • 6

complete list of authors

  • Fluckey, JD||Asp, S||Enevoldsen, LH||Galbo, H

publication date

  • December 2001

publisher