Isolation and sequence analysis of cDNAs for the major potato tuber protein, patatin. Academic Article uri icon

abstract

  • A cDNA library from membrane-bound poly(A)+ mRNA of developing potato tubers was constructed and two classes of essentially full-length cDNA clones for a precursor to the major tuber glycoprotein, patatin were isolated. Sequence analysis shows that the two classes are approximately 99% homologous and correspond to the two major species of patatin identified previously by NH2-terminal amino acid sequence analysis. Sequence analysis also predicts that patatin is synthesized with a 23 amino acid signal sequence. Northern blot analysis shows that patatin mRNAs are 1550 +/- 50 nucleotides in length and are normally not present in polyribosomal or total RNA from stems or leaves.

published proceedings

  • Nucleic Acids Res

altmetric score

  • 9

author list (cited authors)

  • Mignery, G. A., Pikaard, C. S., Hannapel, D. J., & Park, W. D.

citation count

  • 98

complete list of authors

  • Mignery, GA||Pikaard, CS||Hannapel, DJ||Park, WD

publication date

  • January 1984