Isolation and sequence analysis of cDNAs for the major potato tuber protein, patatin. Academic Article

abstract

• A cDNA library from membrane-bound poly(A)+ mRNA of developing potato tubers was constructed and two classes of essentially full-length cDNA clones for a precursor to the major tuber glycoprotein, patatin were isolated. Sequence analysis shows that the two classes are approximately 99% homologous and correspond to the two major species of patatin identified previously by NH2-terminal amino acid sequence analysis. Sequence analysis also predicts that patatin is synthesized with a 23 amino acid signal sequence. Northern blot analysis shows that patatin mRNAs are 1550 +/- 50 nucleotides in length and are normally not present in polyribosomal or total RNA from stems or leaves.

authors

• Park, William

published proceedings

• Nucleic Acids Res

altmetric score

• 9

author list (cited authors)

• Mignery, G. A., Pikaard, C. S., Hannapel, D. J., & Park, W. D.

citation count

• 98

complete list of authors

• Mignery, GA||Pikaard, CS||Hannapel, DJ||Park, WD

publication date

• November 1984

publisher

• Oxford University Press (OUP)  Publisher

published in

• Nucleic Acids Research (NAR)  Journal

keywords

• Amino Acid Sequence
• Animals
• Base Sequence
• Carboxylic Ester Hydrolases
• DNA
• DNA Restriction Enzymes
• Genes
• Nucleic Acid Hybridization
• Plant Proteins
• Plants
• Plasmids
• Poly A
• Polyribosomes
• Protein Biosynthesis
• RNA, Messenger
• Rabbits
• Reticulocytes

PubMed Central ID

• 6150463

Digital Object Identifier (DOI)

• 10.1093/nar/12.21.7987

start page

• 7987

end page

• 8000

volume

• 12

issue

• 21

URL

• http%3A%2F%2Fdx.doi.org%2F10.1093%2Fnar%2F12.21.7987