Production and characterization of monoclonal antibodies against midgut of ixodid tick, Haemaphysalis longicornis. Academic Article

abstract

• There are concerted efforts toward development of tick vaccines to replace current chemical control strategies that have serious limitations [Parasitologia 32 (1990) 145; Infectious Disease Clinics of North America (1999) 209-226]. In this study, monoclonal antibodies (mAbs) specific to Haemaphysalis longicornis midgut proteins were produced and characterized. Eight antibody-secreting hybridomas were cloned and the mAbs typed as IgG1, IgG2a and IgG2b. On immunoblots, all mAbs reacted with a midgut protein band of about 76 kDa. All mAbs uniformly immunogold-stained the surface or epithelial layers of H. longicornis midgut and endosomes. Adult ticks (50%) that fed on an ascitic mouse producing the IgGs developed a red coloration and did not oviposit. As such, the 76 kDa protein that reacted with the mAbs could, therefore, be a potential candidate for tick vaccine development.

authors

• Mulenga, Albert

published proceedings

• Vet Parasitol

author list (cited authors)

• Nakajima, M., Kodama, M., Yanase, H., Iwanaga, T., Mulenga, A., Ohashi, K., & Onuma, M.

citation count

• 3

complete list of authors

• Nakajima, Mie||Kodama, Michi||Yanase, Haruko||Iwanaga, Toshihiko||Mulenga, Albert||Ohashi, Kazuhiko||Onuma, Misao

publication date

• January 2003

publisher

• Elsevier  Publisher

published in

• Veterinary Parasitology  Journal

keywords

• Animals
• Antibodies, Monoclonal
• Blotting, Western
• Hybridomas
• Immunoglobulin Isotypes
• Insect Proteins
• Ixodidae
• Mice
• Mice, Inbred BALB C
• Microscopy, Electron
• Tick Infestations
• Vaccines

Digital Object Identifier (DOI)

• 10.1016/s0304-4017(03)00187-0

start page

• 355

end page

• 363

volume

• 115

issue

• 4

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fs0304-4017%2803%2900187-0

user-defined tag

• 3 Good Health and Well-Being