Production and characterization of monoclonal antibodies against midgut of ixodid tick, Haemaphysalis longicornis. Academic Article uri icon

abstract

  • There are concerted efforts toward development of tick vaccines to replace current chemical control strategies that have serious limitations [Parasitologia 32 (1990) 145; Infectious Disease Clinics of North America (1999) 209-226]. In this study, monoclonal antibodies (mAbs) specific to Haemaphysalis longicornis midgut proteins were produced and characterized. Eight antibody-secreting hybridomas were cloned and the mAbs typed as IgG1, IgG2a and IgG2b. On immunoblots, all mAbs reacted with a midgut protein band of about 76 kDa. All mAbs uniformly immunogold-stained the surface or epithelial layers of H. longicornis midgut and endosomes. Adult ticks (50%) that fed on an ascitic mouse producing the IgGs developed a red coloration and did not oviposit. As such, the 76 kDa protein that reacted with the mAbs could, therefore, be a potential candidate for tick vaccine development.

published proceedings

  • Vet Parasitol

author list (cited authors)

  • Nakajima, M., Kodama, M., Yanase, H., Iwanaga, T., Mulenga, A., Ohashi, K., & Onuma, M.

citation count

  • 3

complete list of authors

  • Nakajima, Mie||Kodama, Michi||Yanase, Haruko||Iwanaga, Toshihiko||Mulenga, Albert||Ohashi, Kazuhiko||Onuma, Misao

publication date

  • August 2003