Stability of an inverted repeat in a human fibrosarcoma cell. Academic Article

abstract

• Deletions and rearrangements of DNA sequences within the genome of human cells result in mutations associated with human disease. We have developed a selection system involving a neo gene containing a DNA sequence inserted into the NcoI site that can be used to quantitatively assay deletion of this sequence from the chromosome. The spontaneous deletion from the neo gene of a 122 bp inverted repeat occurred at a rate of 2.1 x 10(-8) to <3.1 x 10(-9) revertants/cell/generation in three different cell lines. Deletion of the 122 bp inverted repeat occurred between 6 bp flanking direct repeats. Spontaneous deletion of a 122 bp non-palindromic DNA sequence flanked by direct repeats was not observed, indicating a rate of deletion of <3.1 x 10(-9) revertants/cell/generation. This result demonstrates that a 122 bp inverted repeat can exhibit a low level of instability in some locations in the chromosome of a human cell line.

authors

• Kramer, Phillip

published proceedings

• Nucleic Acids Res

author list (cited authors)

• Kramer, P. R., Stringer, J. R., & Sinden, R. R.

citation count

• 9

complete list of authors

• Kramer, PR||Stringer, JR||Sinden, RR

publication date

• November 1996

publisher

• Oxford University Press (OUP)  Publisher

published in

• Nucleic Acids Research (NAR)  Journal

keywords

• Base Sequence
• Cell Line, Transformed
• Chromosome Inversion
• DNA, Recombinant
• Drug Resistance
• Fibrosarcoma
• Humans
• Molecular Sequence Data
• Mutagenesis
• Neomycin
• Plasmids
• Repetitive Sequences, Nucleic Acid
• Restriction Mapping
• Sequence Deletion
• Tumor Cells, Cultured

PubMed Central ID

• 8932378

Digital Object Identifier (DOI)

• 10.1093/nar/24.21.4234

start page

• 4234

end page

• 4241

volume

• 24

issue

• 21

URL

• http://dx.doi.org/10.1093/nar/24.21.4234