A rapid procedure for the isolation of C0t-1 DNA from plants. Academic Article uri icon

abstract

  • In situ hybridization (ISH) for the detection of single- or low-copy sequences, particularly large DNA fragments cloned into YAC or BAC vectors, generally requires the suppression or "blocking" of highly-repetitive DNAs. C0t-1 DNA is enriched for repetitive DNA elements, high or moderate in copy number, and can therefore be used more effectively than total genomic DNA to prehybridize and competitively hybridize repetitive elements that would otherwise cause nonspecific hybridization. C0t-1 DNAs from several mammalian species are commercially available, however, none is currently available for plants to the best of our knowledge. We have developed a simple 1-day procedure to generate C0t-1 DNA without the use of specialized equipment.

published proceedings

  • Genome

altmetric score

  • 3

author list (cited authors)

  • Zwick, M. S., Hanson, R. E., Islam-Faridi, M. N., Stelly, D. M., Wing, R. A., Price, H. J., & McKnight, T. D

citation count

  • 183

complete list of authors

  • Zwick, MS||Hanson, RE||Islam-Faridi, MN||Stelly, DM||Wing, RA||Price, HJ||McKnight, TD

publication date

  • February 1997

published in