Characterization and preliminary mapping of cauliflower mosaic virus transcripts. Academic Article

abstract

• Using Northern blot analysis we have studied the transcription of the CM4-184 Ga. strain of cauliflower mosaic virus (CaMV) DNA. This analysis reveals that this CaMV strain, like the Cabb-BS and the Cabb B-JI strains, produces both a genomic length transcript and a 1900-nucleotide (nt) transcript during infection. In addition, we detect an 1800-nt PA+ transcript mapping primarily to the EcoRI-c region of the virus, and three apparent minor viral-specific PA + RNAs of 4900, 4500, and 4300 nt. We also report the presence of two small viral single-stranded DNAs produced during infection, and show that both DNAs are derived from the EcoRI-b region of the virus.

authors

• Mcknight, Thomas

published proceedings

• Gene

author list (cited authors)

• Condit, C., Hagen, T. J., McKnight, T. D., & Meagher, R. B.

citation count

• 21

complete list of authors

• Condit, C||Hagen, TJ||McKnight, TD||Meagher, RB

publication date

• January 1983

publisher

• Elsevier  Publisher

published in

• Gene  Journal

keywords

• Chromosome Mapping
• Cloning, Molecular
• DNA, Viral
• Electrophoresis, Polyacrylamide Gel
• Mosaic Viruses
• Plants
• RNA, Viral
• Transcription, Genetic

Digital Object Identifier (DOI)

• 10.1016/0378-1119(83)90172-5

start page

• 101

end page

• 108

volume

• 25

issue

• 1

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2F0378-1119%2883%2990172-5