Incomplete entry of bacteriophage T7 DNA into F plasmid-containing Escherichia coli. Academic Article uri icon

abstract

  • The penetration of bacteriophage T7 DNA into F plasmid-containing Escherichia coli cells was determined by measuring Dam methylation of the entering genome. T7 strains that cannot productively infect F-containing cells fail to completely translocate their DNA into the cell before the infection aborts. The entry of the first 44% of the genome occurs normally in an F-containing cell, but the entry of the remainder is aberrant. Bypassing the normal mode of entry of the T7 genome by transfecting naked DNA into competent cells fails to suppress F exclusion of phage development. However, overexpression of various nontoxic T7 1.2 alleles from a high-copy-number plasmid or expression of T3 1.2 from a T7 genome allows phage growth in the presence of F.

altmetric score

  • 3

author list (cited authors)

  • GarcĂ­a, L. R., & Molineux, I. J.

citation count

  • 27

publication date

  • January 1995