Drosophila BAP60 is an essential component of the Brahma complex, required for gene activation and repression.

abstract

• The SWI/SNF-like chromatin remodeling complex of Drosophila, the Brahma complex, contains four subunits (Brahma, BAP155/Moira, SNR1 and BAP60) conserved from yeast to humans. A reconstituted human complex lacking the BAP60 homolog shows full remodeling activity, suggesting that BAP60 is not essential for the core function. We generated Drosophila mutants and found that BAP60 carries a vital function and participates in complex-mediated transcriptional activation and repression. BAP60 binds DNA and shows genetic and physical interactions with the sex-determining transcription factors encoded by sisterless A and scute. The results support the conclusion that BAP60 participates in site-specific recruitment of the Brahma complex in Drosophila.

authors

• Erickson, James

published proceedings

• J Mol Biol

author list (cited authors)

• Mller, A., Avila, F. W., Erickson, J. W., & Jckle, H.

citation count

• 14

complete list of authors

• Möller, André||Avila, Frank W||Erickson, James W||Jäckle, Herbert

publication date

• September 2005

publisher

• Elsevier  Publisher

published in

• Journal of Molecular Biology  Journal

keywords

• Amino Acid Sequence
• Animals
• Basic-Leucine Zipper Transcription Factors
• Cell Cycle Proteins
• DNA-Binding Proteins
• Dosage Compensation, Genetic
• Drosophila Melanogaster
• Drosophila Proteins
• Female
• Gene Expression Regulation, Developmental
• Male
• Molecular Sequence Data
• Mutation
• Protein Binding
• RNA-Binding Proteins
• Sequence Homology, Amino Acid
• Sex Determination Processes
• Trans-Activators
• Transcription Factors
• Transcription, Genetic
• Transcriptional Activation
• X Chromosome

PubMed Central ID

• 16083904

Digital Object Identifier (DOI)

• 10.1016/j.jmb.2005.07.009

start page

• 329

end page

• 337

volume

• 352

issue

• 2

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.jmb.2005.07.009