Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7

abstract

• Mutants of helper phage M13 KO7 were isolated whose survival was up to 300-fold greater than the wild type after 30 min at 95C, and had increased solvent tolerance (up to 200-fold in 75% v/v N,N-dimethylformamide). These mutants retained their ability to package pIII-fusion display phagemids with normal efficiency and therefore will be useful in the isolation of stable protein variants by phage display.

authors

• Benedik, Michael

published proceedings

• BIOTECHNOLOGY LETTERS

author list (cited authors)

• Riddle, R. R., Willson, R. C., & Benedik, M. J.

complete list of authors

• Riddle, RR||Willson, RC||Benedik, MJ

publisher

• Springer Nature  Publisher

published in

• Biotechnology Letters  Journal

keywords

• M13
• Phage Display
• Solvent Tolerance
• Temperature Resistance

Digital Object Identifier (DOI)

• 10.1023/A:1005402209276

start page

• 87

end page

• 90

volume

• 21

issue

• 1