Temperature- and solvent-tolerant mutants of filamentous bacteriophage helper M13 KO7 Academic Article uri icon


  • Mutants of helper phage M13 KO7 were isolated whose survival was up to 300-fold greater than the wild type after 30 min at 95C, and had increased solvent tolerance (up to 200-fold in 75% v/v N,N-dimethylformamide). These mutants retained their ability to package pIII-fusion display phagemids with normal efficiency and therefore will be useful in the isolation of stable protein variants by phage display.

published proceedings


author list (cited authors)

  • Riddle, R. R., Willson, R. C., & Benedik, M. J.

citation count

  • 2

publication date

  • January 1999