A high-throughput droplet microfluidics-based screening platform for quantitative analysis of algal growth and oil accumulation
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14CBMS. We have developed a high-throughput droplet microfluidics-based screening platform for investigating and screening growth and oil production of large numbers of algal strain libraries. Algal growth was characterized by monitoring the number of cells inside droplets, which initially encapsulated a single alga, over time through chlorophyll autofluorescence. The key component of the developed platform is its capability to perform on-chip lipid staining of algae within droplets, where algae-containing droplets and Nile red solution droplets were generated, synchronized, and merged to quantify accumulated oil inside the algae. The performance of this screening platform was demonstrated using unicellular alga Chlamydomonas reinhardtii.