Cellular effects induced by the antitumor agent azinomycin B. Academic Article

abstract

• Studies on the mechanism of action of the antitumor agent azinomycin B in vitro suggest that the drug elicits its lethal effects by the formation of interstrand crosslinks within the major groove of DNA. Here, we demonstrate the biological effects of the drug in vivo. Fluorescence imaging revealed localization of azinomycin B in the nuclear region of yeast. Moreover, experiments with oligonucleotide microarrays examined the effects of the drug across the yeast transcriptome. The results demonstrated a robust DNA damage response that supports the proposed role of the drug as a covalent DNA modifying agent. RT-PCR analysis validated the gene changes, and flow cytometry of azinomycin-treated yeast cells demonstrated a phenotypic S phase shift consistent with transcriptional effects.

authors

• Smith, Roger
• Watanabe, Coran

published proceedings

• Chem Biol

author list (cited authors)

• Kelly, G. T., Liu, C., Smith, R., Coleman, R. S., & Watanabe, C.

citation count

• 23

complete list of authors

• Kelly, Gilbert T||Liu, Chaomin||Smith, Roger||Coleman, Robert S||Watanabe, Coran MH

publication date

• May 2006

publisher

• Elsevier  Publisher

published in

• Chemistry and Biology  Journal

keywords

• Alkylation
• Antineoplastic Agents
• Base Sequence
• Cell Cycle
• DNA Damage
• DNA Primers
• DNA, Fungal
• Intercellular Signaling Peptides And Proteins
• Naphthalenes
• Peptides
• Reverse Transcriptase Polymerase Chain Reaction
• Saccharomyces Cerevisiae
• Transcription, Genetic

PubMed Central ID

• 16720269

Digital Object Identifier (DOI)

• 10.1016/j.chembiol.2006.02.015

start page

• 485

end page

• 492

volume

• 13

issue

• 5

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.chembiol.2006.02.015