Initial characterization of a type I fatty acid synthase and polyketide synthase multienzyme complex NorS in the biosynthesis of aflatoxin B(1). Academic Article

abstract

• The biosynthesis of the potent environmental carcinogen aflatoxin B(1) is initiated by norsolorinic acid synthase (NorS), a complex of an iterative type I polyketide synthase and a specialized yeast-like pair of fatty acid synthases. NorS has been partially purified from Aspergillus parasiticus, has been found to have a mass of approximately 1.4 x 10(6) Da, and carries out the synthesis of norsolorinic acid in the presence of acetylCoA, malonylCoA, and NADPH where hexanoylCoA is not a free intermediate. The N-acetylcysteamine thioester of hexanoic acid can substitute for the catalytic functions of HexA/B to initiate norsolorinic acid synthesis by the complex in the presence of only malonylCoA. An alpha(2)beta(2)gamma(2) stoichiometry is proposed for NorS in keeping with its estimated mass and the observed dimeric or higher-order quarternary structures of PKS and FAS enzymes.

authors

• Watanabe, Coran

published proceedings

• Chem Biol

altmetric score

• 6

author list (cited authors)

• Watanabe, C., & Townsend, C. A.

citation count

• 84

complete list of authors

• Watanabe, Coran MH||Townsend, Craig A

publication date

• January 2002

publisher

• Elsevier  Publisher

published in

• Chemistry and Biology  Journal

keywords

• Aflatoxin B1
• Anthraquinones
• Aspergillus
• Autoradiography
• Caproates
• Chromatography, High Pressure Liquid
• Coenzyme A
• Electrophoresis, Polyacrylamide Gel
• Fatty Acid Synthases
• Multienzyme Complexes
• NADP
• Sulfhydryl Compounds

PubMed Central ID

• 12323372

Digital Object Identifier (DOI)

• 10.1016/s1074-5521(02)00213-2

start page

• 981

end page

• 988

volume

• 9

issue

• 9

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fs1074-5521%2802%2900213-2