Characterization of AziR, a resistance protein of the DNA cross-linking agent azinomycin B. Academic Article

abstract

• A protein identified from the Streptomyces sahachiroi genome exhibits a protective effect against the DNA alkylator azinomycin B when heterologously expressed in S. lividans and E. coli. The protein, dubbed AziR for azinomycin resistance, is homologous to aminoglycoside phosphotransferases but behaves as an azinomycin binding protein and fails to chemically modify azinomycin. While AziR confers resistance to azinomycin B, it is inactive against aminoglycoside antibiotics and other DNA alkylators. A nucleic acid staining assay indicates that the protein enhances cell survival, and also prevents DNA damage effects normally observed following azinomycin treatment. Knowledge of an azinomycin resistance mechanism aids in setting the stage for future engineered biosynthesis of functionally useful azinomycin analogues.

authors

• Watanabe, Coran

author list (cited authors)

• Foulke-Abel, J., Kelly, G. T., Zhang, H., & Watanabe, C

citation count

• 10

complete list of authors

• Foulke-Abel, Jennifer||Kelly, Gilbert T||Zhang, Huitu||Watanabe, Coran MH

publication date

• January 2011

publisher

• Royal Society of Chemistry (RSC)  Publisher

published in

• Molecular Biosystems  Journal

keywords

• Anti-Bacterial Agents
• Bacterial Proteins
• Carrier Proteins
• Cross-Linking Reagents
• Escherichia Coli
• Intercellular Signaling Peptides And Proteins
• Naphthalenes
• Peptides
• Streptomyces

PubMed Central ID

• 21776524

Digital Object Identifier (DOI)

• 10.1039/c1mb05136a

start page

• 2563

end page

• 2570

volume

• 7

issue

• 9

URL

• http%3A%2F%2Fdx.doi.org%2F10.1039%2Fc1mb05136a