A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli.
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By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.
author list (cited authors)
Huang, Y., Russell, W. K., Wan, W., Pai, P., Russell, D. H., & Liu, W.
complete list of authors
Huang, Ying||Russell, William K||Wan, Wei||Pai, Pei-Jing||Russell, David H||Liu, Wenshe