A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli Academic Article uri icon


  • By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.

altmetric score

  • 6

author list (cited authors)

  • Huang, Y., Russell, W. K., Wan, W., Pai, P., Russell, D. H., & Liu, W.

citation count

  • 50

publication date

  • February 2010