A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli. Academic Article

abstract

• By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.

authors

• Liu, Wenshe
• Russell, David

published proceedings

• Mol Biosyst

altmetric score

• 6

author list (cited authors)

• Huang, Y., Russell, W. K., Wan, W., Pai, P., Russell, D. H., & Liu, W.

citation count

• 54

complete list of authors

• Huang, Ying||Russell, William K||Wan, Wei||Pai, Pei-Jing||Russell, David H||Liu, Wenshe

publication date

• April 2010

publisher

• Royal Society of Chemistry (RSC)  Publisher

published in

• Molecular Biosystems  Journal

keywords

• Amino Acid Sequence
• Amino Acids
• Base Sequence
• Codon, Terminator
• DNA, Bacterial
• Escherichia Coli
• Escherichia Coli Proteins
• Genes, Bacterial
• Green Fluorescent Proteins
• Lysine
• Models, Molecular
• Molecular Sequence Data
• Protein Processing, Post-Translational
• Recombinant Fusion Proteins
• Ribosomal Proteins
• Spectrometry, Mass, Electrospray Ionization
• Systems Biology

PubMed Central ID

• 20237646

Digital Object Identifier (DOI)

• 10.1039/b920120c

start page

• 683

end page

• 686

volume

• 6

issue

• 4