A convenient method for genetic incorporation of multiple noncanonical amino acids into one protein in Escherichia coli. Academic Article uri icon

abstract

  • By overexpressing the C-terminal domain of the ribosomal protein L11 to decrease release factor 1-mediated termination of protein translation, enhanced amber suppression is achieved in E. coli. This enhanced amber suppression efficiency allows the genetic incorporation of three N(epsilon)-acetyl-l-lysines into one GFP(UV) protein in E. coli.

published proceedings

  • Mol Biosyst

altmetric score

  • 6

author list (cited authors)

  • Huang, Y., Russell, W. K., Wan, W., Pai, P., Russell, D. H., & Liu, W.

citation count

  • 52

complete list of authors

  • Huang, Ying||Russell, William K||Wan, Wei||Pai, Pei-Jing||Russell, David H||Liu, Wenshe

publication date

  • April 2010