A genetically encoded photocaged Nepsilon-methyl-L-lysine.
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A photocaged N(epsilon)-methyl-L-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover N(epsilon)-methyl-L-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.
author list (cited authors)
Wang, Y., Wu, B. o., Wang, Z., Huang, Y., Wan, W., Russell, W. K., ... Liu, W. R.
complete list of authors
Wang, Yane-Shih||Wu, Bo||Wang, Zhiyong||Huang, Ying||Wan, Wei||Russell, William K||Pai, Pei-Jing||Moe, Yin N||Russell, David H||Liu, Wenshe R