A genetically encoded photocaged Nepsilon-methyl-L-lysine. Academic Article uri icon

abstract

  • A photocaged N(epsilon)-methyl-L-lysine has been genetically incorporated into proteins at amber codon positions in Escherichia coli using an evolved pyrrolysyl-tRNA synthetase-pylT pair. Its genetic incorporation and following photolysis to recover N(epsilon)-methyl-L-lysine at physiological pH provide a convenient method for the biosynthesis of proteins with monomethylated lysines at specific sites.

published proceedings

  • Mol Biosyst

altmetric score

  • 0.5

author list (cited authors)

  • Wang, Y., Wu, B. o., Wang, Z., Huang, Y., Wan, W., Russell, W. K., ... Liu, W. R.

citation count

  • 66

complete list of authors

  • Wang, Yane-Shih||Wu, Bo||Wang, Zhiyong||Huang, Ying||Wan, Wei||Russell, William K||Pai, Pei-Jing||Moe, Yin N||Russell, David H||Liu, Wenshe R

publication date

  • September 2010