A genetically encoded aldehyde for rapid protein labelling
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Using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.
author list (cited authors)
Tuley, A., Lee, Y., Wu, B. o., Wang, Z. U., & Liu, W. R.