A genetically encoded aldehyde for rapid protein labelling Academic Article uri icon

abstract

  • Using a mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pair, 3-formyl-phenylalanine is genetically incorporated into proteins at amber mutation sites in Escherichia coli. This non-canonical amino acid readily reacts with hydroxylamine dyes, leading to rapid and site-selective protein labelling.

author list (cited authors)

  • Tuley, A., Lee, Y., Wu, B. o., Wang, Z. U., & Liu, W. R.

citation count

  • 24

publication date

  • July 2014