A facile system for genetic incorporation of two different noncanonical amino acids into one protein in Escherichia coli.
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abstract
Two's company: Using a wild-type or evolved PylRS-pylTUUA pair to suppress ochre mutation and an evolved MjTyrRSMjtRNACUATyr pair to suppress amber mutation, two different noncanonical amino acids (NAAs) have been concomitantly incorporated into one protein in E. coli with high efficiency (see picture, with NAAs 1-4; GFP = green-fluorescent protein). "Chemical equation presented" 2010 Wiley-VCH Verlag GmbH & Co. KGaA.