Genetically encoded fluorophenylalanines enable insights into the recognition of lysine trimethylation by an epigenetic reader. Academic Article uri icon

abstract

  • Fluorophenylalanines bearing 2-5 fluorine atoms at the phenyl ring have been genetically encoded by amber codon. Replacement of F59, a phenylalanine residue that is directly involved in interactions with trimethylated K9 of histone H3, in the Mpp8 chromodomain recombinantly with fluorophenylalanines significantly impairs the binding to a K9-trimethylated H3 peptide.

author list (cited authors)

  • Lee, Y., Schmidt, M. J., Tharp, J. M., Weber, A., Koenig, A. L., Zheng, H., ... Liu, W. R

complete list of authors

  • Lee, Yan-Jiun||Schmidt, MJ||Tharp, Jeffery M||Weber, Annemarie||Koenig, Amber L||Zheng, Hong||Gao, Jianmin||Waters, Marcey L||Summerer, Daniel||Liu, Wenshe R