An efficient enzymatic synthesis of thiamin pyrophosphate.

abstract

• Thiamin pyrophosphate was synthesized in 71% yield, on a multi-milligram scale, using overexpressed thiazole kinase, pyrimidine kinase, thiamin phosphate synthase, and thiamin phosphate kinase. This provides a facile route to isotopically labeled thiamin pyrophosphate from its readily available pyrimidine and thiazole precursors.

authors

• Begley, Tadhg

published proceedings

• Bioorg Med Chem Lett

author list (cited authors)

• Melnick, J. S., Sprinz, K. I., Reddick, J. J., Kinsland, C., & Begley, T. P.

citation count

• 9

complete list of authors

• Melnick, Jonathan S||Sprinz, K Ingrid||Reddick, Jason J||Kinsland, Cynthia||Begley, Tadhg P

publication date

• January 2003

publisher

• Elsevier  Publisher

published in

• Bioorganic and Medicinal Chemistry Letters  Journal

keywords

• Base Sequence
• Cloning, Molecular
• DNA Primers
• Escherichia Coli
• Phosphotransferases (phosphate Group Acceptor)
• Polymerase Chain Reaction
• Recombinant Proteins
• Thiamine Pyrophosphate

Digital Object Identifier (DOI)

• 10.1016/j.bmcl.2003.07.026

start page

• 4139

end page

• 4141

volume

• 13

issue

• 22

URL

• http%3A%2F%2Fdx.doi.org%2F10.1016%2Fj.bmcl.2003.07.026