An efficient enzymatic synthesis of thiamin pyrophosphate. Academic Article uri icon


  • Thiamin pyrophosphate was synthesized in 71% yield, on a multi-milligram scale, using overexpressed thiazole kinase, pyrimidine kinase, thiamin phosphate synthase, and thiamin phosphate kinase. This provides a facile route to isotopically labeled thiamin pyrophosphate from its readily available pyrimidine and thiazole precursors.

published proceedings

  • Bioorg Med Chem Lett

author list (cited authors)

  • Melnick, J. S., Sprinz, K. I., Reddick, J. J., Kinsland, C., & Begley, T. P.

citation count

  • 9

complete list of authors

  • Melnick, Jonathan S||Sprinz, K Ingrid||Reddick, Jason J||Kinsland, Cynthia||Begley, Tadhg P

publication date

  • January 2003