An efficient enzymatic synthesis of thiamin pyrophosphate.
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Thiamin pyrophosphate was synthesized in 71% yield, on a multi-milligram scale, using overexpressed thiazole kinase, pyrimidine kinase, thiamin phosphate synthase, and thiamin phosphate kinase. This provides a facile route to isotopically labeled thiamin pyrophosphate from its readily available pyrimidine and thiazole precursors.
author list (cited authors)
Melnick, J. S., Sprinz, K. I., Reddick, J. J., Kinsland, C., & Begley, T. P.
complete list of authors
Melnick, Jonathan S||Sprinz, K Ingrid||Reddick, Jason J||Kinsland, Cynthia||Begley, Tadhg P