Mechanistic studies on prolyl-4-hydroxylase: the vitamin C requiring uncoupled oxidation. Academic Article uri icon


  • A deuterated substrate for the human type I prolyl-4-hydroxylase was synthesized and its V/K deuterium isotope effect was determined to be 3.4 +/- 0.2. This isotope effect was attributed to the uncoupled oxidation. A dehydroproline containing tetrapeptide was also found to stimulate the uncoupled oxidation.

published proceedings

  • Bioorg Med Chem Lett

author list (cited authors)

  • Wu, M., Moon, H. S., Pirskanen, A., Myllyharju, J., Kivirikko, K. I., & Begley, T. P.

citation count

  • 9

complete list of authors

  • Wu, M||Moon, HS||Pirskanen, A||Myllyharju, J||Kivirikko, KI||Begley, TP

publication date

  • January 2000