Mechanistic studies on prolyl-4-hydroxylase: the vitamin c requiring uncoupled oxidation Academic Article uri icon

abstract

  • A deuterated substrate for the human type I prolyl-4-hydroxylase was synthesized and its V/K deuterium isotope effect was determined to be 3.4 +/- 0.2. This isotope effect was attributed to the uncoupled oxidation. A dehydroproline containing tetrapeptide was also found to stimulate the uncoupled oxidation.

author list (cited authors)

  • Wu, M., Moon, H., Pirskanen, A., Myllyharju, J., Kivirikko, K. I., & Begley, T. P.

citation count

  • 9

publication date

  • July 2000