Homogenous enzymatic synthesis using a thermo-responsive water-soluble polymer support Academic Article uri icon


  • Several enzymes immobilized on thermoresponsive polyacrylamide polymers are nearly as active as their soluble forms, and can be recovered for reuse after gentle heating and precipitation. Carbohydrates attached to these polymers have been used for enzymatic glycoslation, and the products have been isolated by thermal precipitation followed by release from the polymer, thus greatly simplifying product purification in water.

published proceedings


author list (cited authors)

  • Huang, X. F., Witte, K. L., Bergbreiter, D. E., & Wong, C. H.

complete list of authors

  • Huang, XF||Witte, KL||Bergbreiter, DE||Wong, CH

publication date

  • August 2001