Homogenous enzymatic synthesis using a thermo-responsive water-soluble polymer support Academic Article

abstract

• Several enzymes immobilized on thermoresponsive polyacrylamide polymers are nearly as active as their soluble forms, and can be recovered for reuse after gentle heating and precipitation. Carbohydrates attached to these polymers have been used for enzymatic glycoslation, and the products have been isolated by thermal precipitation followed by release from the polymer, thus greatly simplifying product purification in water.

authors

• Bergbreiter, David

published proceedings

• ADVANCED SYNTHESIS & CATALYSIS

author list (cited authors)

• Huang, X. F., Witte, K. L., Bergbreiter, D. E., & Wong, C. H.

complete list of authors

• Huang, XF||Witte, KL||Bergbreiter, DE||Wong, CH

publication date

• August 2001

published in

• Advanced Synthesis and Catalysis  Journal

keywords

• Enzyme Immobilization
• Glycosylations
• Polymers
• Thermo-responsive

start page

• 675

end page

• 681

volume

• 343

issue

• 6-7