Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules. Chapter

abstract

• The Golgi complex is the Grand Central Station of intracellular membrane trafficking in the secretory and endocytic pathways. Anterograde and retrograde export of cargo from the Golgi complex involves a complex interplay between the formation of coated vesicles and membrane tubules, although much less is known about tubule-mediated trafficking. Recent advances using in vitro assays have identified several cytoplasmic phospholipase A2 (PLA2) enzymes that are required for the biogenesis of membrane tubules and their roles in the functional organization of the Golgi complex. In this chapter we describe methods for the cell-free reconstitution of PLA2-dependent Golgi membrane tubule formation. These methods should facilitate the identification of other proteins that regulate this process.

authors

• de Figueiredo, Paul

author list (cited authors)

• Cluett, E. B., de Figueiredo, P., Bechler, M. E., Thorsen, K. D., & Brown, W. J.

citation count

• 2

complete list of authors

• Cluett, Edward B||de Figueiredo, Paul||Bechler, Marie E||Thorsen, Kevin D||Brown, William J

Book Title

• GOLGI COMPLEX: METHODS AND PROTOCOLS

publication date

• January 2016

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Golgi Apparatus
• Golgi Complex
• Humans
• In Vitro Reconstitution
• Intracellular Membranes
• Liver
• Membrane Tubules
• Phospholipases A2
• Pla2 Enzymes
• Rats

PubMed Central ID

• 27632003

Digital Object Identifier (DOI)

• 10.1007/978-1-4939-6463-5_7

International Standard Book Number (ISBN) 13

• 978-1-4939-6461-1

start page

• 75

end page

• 90

volume

• 1496

URL

• http%3A%2F%2Fdx.doi.org%2F10.1007%2F978-1-4939-6463-5_7