PCR amplification of microsatellites from single cells of Karenia brevis preserved in Lugol's iodine solution. Academic Article

abstract

• A simple and effective protocol is described for multiplex polymerase chain reaction (PCR) amplification of single cells of Karenia brevis. The protocol requires minimum processing, avoids additions that might dilute target DNA template, and can be used on cells preserved in Lugol's iodine preservative. Destaining of Lugol's-preserved cells with sodium thiosulfate allowed successful amplification of single-copy, nuclear-encoded microsatellites in single cells of K. brevis that have been preserved for up to 6 years.

authors

• Campbell, Lisa
• Henrichs, Darren

published proceedings

• Mar Biotechnol (NY)

author list (cited authors)

• Henrichs, D. W., Renshaw, M. A., Santamaria, C. A., Richardson, B., Gold, J. R., & Campbell, L.

citation count

• 14

complete list of authors

• Henrichs, DW||Renshaw, MA||Santamaria, CA||Richardson, B||Gold, JR||Campbell, L

publication date

• March 2008

publisher

• Springer Nature  Publisher

published in

• Marine Biotechnology  Journal

keywords

• Alleles
• Animals
• Dinoflagellida
• Genotype
• Iodides
• Microsatellite Repeats
• Polymerase Chain Reaction
• Preservation, Biological

Digital Object Identifier (DOI)

• 10.1007/s10126-007-9044-y

start page

• 122

end page

• 127

volume

• 10

issue

• 2

URL

• http://dx.doi.org/10.1007/s10126-007-9044-y