The gag-mos hybrid protein of ts110 Moloney murine sarcoma virus: variation of gene expression with temperature.
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abstract
A NRK cell clone (6m2 cells) infected with ts110 Moloney murine sarcoma virus (MuSV) produce a gag-mos protein, P85gag-mos, and a truncated gag protein of Mr 58,000d termed P58gag. The gag-mos protein is produced from a 3.5-kb mRNA whereas the gag protein is made from a 4.0-kb mRNA. It has been proposed that the 3.5-kb RNA is produced from the 4.0-kb RNA by a splicing mechanism (R. P. Junghans, E. C. Murphy, Jr., and R. B. Arlinghaus (1982) J. Mol. Biol. 161, 229-255). The results presented here provide further support for this model. The expression of the 3.5-kb RNA and the gag-mos protein increased as the temperature at which 6m2 cells were maintained was lowered from 39 to 28 degrees. This increase coincided with a decrease in both the 4.0-kb RNA and its product P58gag. The optimum temperature for syntheses of both the gag-mos mRNA and its protein was found to be 28 degrees. Consistent with the increase in the level of the gag-mos protein is the increase in the protein kinase activity associated with P85gag-mos and the degree of morphological transformation of 6m2 cells. Thus, the level of P85gag-mos within 6m2 cells is directly proportional to the degree of cell transformation and the amount of the kinase activity associated with the gag-mos protein, providing convincing evidence that P85gag-mos plays a direct role in the neoplastic transformation of these cells.
