McKee, Kyle Patrick (2015-02). Biological Manganese Oxidation by Pseudomonas putida in Trickling Filters. Master's Thesis. Thesis uri icon


  • Manganese (Mn) is considered a nuisance chemical in drinking water. Manganese causes problems with staining, foul odor, undesirable tastes, and can be corrosive to pipelines. The United States Environmental Protection Agency (US EPA) recommends a secondary maximum contaminant level for Mn below a concentration of 0.05 mg/L. Currently manganese contaminated water is typically treated using expensive and potentially harmful oxidizing agents. Biological treatment techniques have been researched as a viable alternative for removing undesired chemicals from drinking water. In this study, bench scale trickling filters were constructed to compare the Mn removal efficiency between biochemical and abiotic processes. Glass beads between three and five millimeters in diameter were used as the solid media in the trickling filters with and without inoculation of a Mn oxidizing bacterium, Pseudomonas putida. Manganese oxidation and removal was found to be significantly greater in trickling filters with Pseudomonas putida biofilms after startup times of only 48 hours. Mn oxidation in Pseudomonas putida inoculated trickling filters was up to 75% greater than non-inoculated filters. One dimensional advection dispersive models were formulated to describe the transport of Mn in trickling filter porous media. Using the data collected in the experiments, the model predicted that that an average of 10 mg/L of influent Mn (II) concentration can be decreased by 78.56% with a filter depth of only 10 cm. The rapid startup time and the high Mn removal capacity of trickling filters inoculated with Pseudomonas putida can potentially become a mainstream treatment system in conjunction with sand filters.

publication date

  • May 2015