Turk, Stacey N. (2008-05). Fatty Acid Carcass Mapping. Master's Thesis. Thesis uri icon

abstract

  • We hypothesized that subcutaneous (s.c.) adipose tissue would differ in monounsaturated (MUFA) and saturated fatty acid (SFA) composition among different depots throughout a beef carcass. To test this, 50 carcasses from a variety of breed types and backgrounds were sampled. External fat samples were collected from eight different carcass locations: round, sirloin, loin, rib, chuck, brisket, plate and flank. Samples were used to provide information on slip points, fatty acid composition and MUFA:SFA ratios. Lipids were extracted from s.c. adipose tissue by a modified chloroform:methanol procedure, and fatty acid composition and slip points were measured. The brisket was significantly lower in palmitic (16:0) and stearic (18:0) acid than the other seven sampling sites (P = 0.001). The brisket demonstrated the highest values of MUFA (P = 0.001) with the exception of possessing the lowest value of transvaccenic (18:1t11) acid (P = 0.002). There were also significant differences in the amounts of PUFA among the eight sampling sites. The lowest values were from the brisket with a mean of 25.1. The flank had the highest slip point with a mean of 39.0 (P < or = 0.001). There was a high negative correlation shown between palmitoleic and stearic acid (R2 = 0.827). The brisket displayed the highest values for MUFA:SFA ratios (P = 0.001), whereas the flank was the lowest. Due to the significant differences amongst fat depots within bovine carcasses in their fatty acid composition we conclude that substantial differences exist across fat depots.
  • We hypothesized that subcutaneous (s.c.) adipose tissue would differ in

    monounsaturated (MUFA) and saturated fatty acid (SFA) composition among different

    depots throughout a beef carcass. To test this, 50 carcasses from a variety of breed types

    and backgrounds were sampled. External fat samples were collected from eight different

    carcass locations: round, sirloin, loin, rib, chuck, brisket, plate and flank. Samples were

    used to provide information on slip points, fatty acid composition and MUFA:SFA

    ratios. Lipids were extracted from s.c. adipose tissue by a modified

    chloroform:methanol procedure, and fatty acid composition and slip points were

    measured. The brisket was significantly lower in palmitic (16:0) and stearic (18:0) acid

    than the other seven sampling sites (P = 0.001). The brisket demonstrated the highest

    values of MUFA (P = 0.001) with the exception of possessing the lowest value of transvaccenic

    (18:1t11) acid (P = 0.002). There were also significant differences in the

    amounts of PUFA among the eight sampling sites. The lowest values were from the

    brisket with a mean of 25.1. The flank had the highest slip point with a mean of 39.0

    (P < or = 0.001). There was a high negative correlation shown between palmitoleic and

    stearic acid (R2 = 0.827). The brisket displayed the highest values for MUFA:SFA ratios (P = 0.001), whereas the flank was the lowest. Due to the significant differences

    amongst fat depots within bovine carcasses in their fatty acid composition we conclude

    that substantial differences exist across fat depots.

publication date

  • May 2008