2-D nanomaterials have received much attention as building blocks in energy-related devices and applications. The increasing demand for those nanomaterials has brought along environmental concerns at the same time. Due to their unique thermal, mechanical, and interfacial properties, such nanomaterials constitute unknown principles in the context of nanosafety, nanotoxicity, and the environmental effects to the entire ecosystem of the earth. The research to know the science of those nanomaterials is required, as disposal of such chemicals can be toxic to the environment. In this thesis, the concise mechanism of surface adhesion of 2-D nanomaterials to soil bacteria cells will be reviewed. In addition, from the relationship between microorganisms and the entire soil environment system, we can predict the environmental impacts of the mass disposal of those nanomaterials systemically. Experiments were conducted to investigate the influence of 2-D nanosheets of Molybdenum Disulfide (MoS2) to the survivability of soil bacteria dissolved in deionized water. Gram positive bacteria, Bacillus cereus will be released to the several different concentrations of MoS2 nanosheets in a 24-hour timeline, to examine the cytotoxicity of these nanoparticles in pure water. The entire procedure can be set as a standard to assess the toxicity of any other energy-related 2-D nanomaterials to the other gram positive and negative bacteria for further research.