Garcia-Uribe, Alejandro (2009-05). NON-INVASIVE OPTICAL DETECTION OF EPITHELIAL CANCER USING OBLIQUE INCIDENCE DIFFUSE REFLECTANCE SPECTROSCOPY. Doctoral Dissertation. Thesis uri icon

abstract

  • This dissertation describes the design, fabrication and testing of an oblique incidence diffuse reflectance spectrometry (OIDRS) system for in-vivo and noninvasive detection of epithelial cancer. Two probes were fabricated using micromachining technology, which plays a significant role in the probe development by enabling device miniaturization, low-cost fabrication and precise assembly. The fist probe was developed and clinically tested for skin cancer detection. This probe consists of three source fibers, two linear array of collection fibers and four micromachined positioning devices for accurate alignment of the fibers. The spatially resolved diffuse reflectance spectra from 167 pigmented and 78 non-pigmented skin abnormalities were measured and used to design a set of classifiers to separate them into benign or malignant ones. These classifiers perform with an overall classification rate of 91%. The absorption and reduced scattering coefficient spectra were estimated to link the anatomic and physiologic properties of the lesions with the optical diagnosis. The melanoma cases presented larger average absorption and reduced scattering spectra than the dysplastic and benign ones. A second probe was designed to demonstrate the feasibility of a miniaturized ?side viewing? optical sensor probe for OIDRS. The sensor probe consists of a lithographically patterned polymer waveguides chip and two micromachined positioning substrates. This miniaturize probe was used to measure twenty ex-vivo esophageal samples. Two statistical classifiers were designed to separate the esophageal cases. The first one distinguishes benign and low dysplastic from high dysplastic and cancerous lesions. The second classifier separates benign lesions from low dysplastic ones. Both classifiers generated a classification rate of 100%.
  • This dissertation describes the design, fabrication and testing of an oblique incidence
    diffuse reflectance spectrometry (OIDRS) system for in-vivo and noninvasive detection
    of epithelial cancer. Two probes were fabricated using micromachining technology,
    which plays a significant role in the probe development by enabling device
    miniaturization, low-cost fabrication and precise assembly. The fist probe was developed
    and clinically tested for skin cancer detection. This probe consists of three source fibers,
    two linear array of collection fibers and four micromachined positioning devices for
    accurate alignment of the fibers. The spatially resolved diffuse reflectance spectra from
    167 pigmented and 78 non-pigmented skin abnormalities were measured and used to
    design a set of classifiers to separate them into benign or malignant ones. These
    classifiers perform with an overall classification rate of 91%. The absorption and
    reduced scattering coefficient spectra were estimated to link the anatomic and
    physiologic properties of the lesions with the optical diagnosis. The melanoma cases
    presented larger average absorption and reduced scattering spectra than the dysplastic
    and benign ones. A second probe was designed to demonstrate the feasibility of a miniaturized ?side viewing? optical sensor probe for OIDRS. The sensor probe consists
    of a lithographically patterned polymer waveguides chip and two micromachined
    positioning substrates. This miniaturize probe was used to measure twenty ex-vivo
    esophageal samples. Two statistical classifiers were designed to separate the esophageal
    cases. The first one distinguishes benign and low dysplastic from high dysplastic and
    cancerous lesions. The second classifier separates benign lesions from low dysplastic
    ones. Both classifiers generated a classification rate of 100%.

publication date

  • May 2009