Arenas Gamboa, Angela Maria (2007-05). Evaluation of microencapsulation as an improved vaccination strategy against brucellosis. Doctoral Dissertation. Thesis uri icon

abstract

  • Brucellosis is an important zoonotic disease of nearly worldwide distribution. Despite the availability of live vaccine strains for bovine (S19, RB51) and small ruminants (Rev 1), these vaccines have several drawbacks including residual virulence for animals and humans. Safe and efficacious immunization systems are therefore needed to overcome these disadvantages. Brucella melitensis and Brucella abortus mutants in the luxR gene were generated and investigated for theri potential use as improve vaccine candidates. Immunization with a sustained release vehicle to enhance vaccination efficacy was evaluated utilizing the live mutants in encapsulated alginate microspheres containing a non-immunogenic eggshell precursor protein of the parasite Fasciola hepatica (Vitelline protein B, VpB). BALB/c mice were immunized with either encapsulated or nonencapsulated vaccine candidates to evaluate immunogenicity, safety and protective efficacy. The results suggest that luxR mutants, are attenuated in the mouse and macrophage model and appear good and safe vaccine candidates when the immunogen is given in a microencapsulated format. We were also able to demonstrate the utility of microencapsulation in oral delivery by increasing vaccine performance of current licensed vaccine strains in a natural host, the Red Deer. Together, these results suggest that microencapsulation of live Brucella produces an enhanced delivery vaccine system against brucellosis increasing the efficacy of poorly-performing nonencapsulated vaccine candidates.
  • Brucellosis is an important zoonotic disease of nearly worldwide distribution.
    Despite the availability of live vaccine strains for bovine (S19, RB51) and small
    ruminants (Rev 1), these vaccines have several drawbacks including residual virulence
    for animals and humans. Safe and efficacious immunization systems are therefore
    needed to overcome these disadvantages. Brucella melitensis and Brucella abortus
    mutants in the luxR gene were generated and investigated for theri potential use as
    improve vaccine candidates. Immunization with a sustained release vehicle to enhance
    vaccination efficacy was evaluated utilizing the live mutants in encapsulated alginate
    microspheres containing a non-immunogenic eggshell precursor protein of the parasite
    Fasciola hepatica (Vitelline protein B, VpB). BALB/c mice were immunized with either
    encapsulated or nonencapsulated vaccine candidates to evaluate immunogenicity,
    safety and protective efficacy. The results suggest that luxR mutants, are attenuated in
    the mouse and macrophage model and appear good and safe vaccine candidates when
    the immunogen is given in a microencapsulated format. We were also able to
    demonstrate the utility of microencapsulation in oral delivery by increasing vaccine
    performance of current licensed vaccine strains in a natural host, the Red Deer. Together, these results suggest that microencapsulation of live Brucella
    produces an enhanced delivery vaccine system against brucellosis increasing the
    efficacy of poorly-performing nonencapsulated vaccine candidates.

publication date

  • May 2007