Longenberger, Polly Suzanne (2008-05). Evaluation of chlorophyll fluorescence as a tool for the identification of drought tolerance in upland cotton. Doctoral Dissertation. Thesis uri icon

abstract

  • A novel bioassay for the evaluation of plant water status was developed by Burke (2007). The research reported herein was designed to evaluate this new protocol as a tool for use in cotton breeding programs for the identification of drought tolerant genotypes. Twenty genotypes were selected to represent diverse germplasm pools for a two-year field evaluation. Replicated tests were performed in Lubbock, TX and College Station, TX in 2005, 2006, and 2007. Dryland and irrigated treatments were administered in a split plot arrangement of a randomized complete block design. Fluorescence measurements were taken at mid-bloom and late bloom growth stages of growth. Source leaf tissue was harvested at predawn and subjected to high temperature incubation with fluorescence measurements subsequently taken hourly for five hours. Drought stressed plants had not mobilized their carbohydrate reserves from their source leaves overnight and thus maintained cell viability and therefore higher chlorophyll fluorescence values throughout the incubation with the opposite being true for nonstressed plants. Fiber lint yield and fiber properties were measured at the conclusion of the 2005 season in College Station and the 2006 season in College Station and Lubbock for comparison with the fluorescence data. Five genotypes, 'Acala 1517-99', 'Deltapine 491' (PVP no. 200100159), 'Tamcot CAMD-E', 'Tamcot 22' and TAM 89E-51, an unreleased breeding line, were selected based on field evaluation results in a preliminary study in 2005 to be included in a diallel analysis to determine the heritability of fluorescence measurements. Genotype x treatment effects complicated the classification of genotypic responses to drought. Few and inconsistent correlations were found among fluorescence values and lint yield or fiber properties. The diallel analysis did not identify general combining ability or specific combining ability effects for chlorophyll fluorescence measurements. Thus this procedure provides little potential in selecting plants for drought tolerance when plants are grown under field culture. Selection among Tamcot 22 and TAM 89E-51 plants for high and low genotypes according to fluorescence values did not yield progeny different from unselected Tamcot 22 and TAM 89E-51.
  • A novel bioassay for the evaluation of plant water status was developed by Burke
    (2007). The research reported herein was designed to evaluate this new protocol as a
    tool for use in cotton breeding programs for the identification of drought tolerant
    genotypes. Twenty genotypes were selected to represent diverse germplasm pools for a
    two-year field evaluation. Replicated tests were performed in Lubbock, TX and College
    Station, TX in 2005, 2006, and 2007. Dryland and irrigated treatments were
    administered in a split plot arrangement of a randomized complete block design.
    Fluorescence measurements were taken at mid-bloom and late bloom growth stages of
    growth. Source leaf tissue was harvested at predawn and subjected to high temperature
    incubation with fluorescence measurements subsequently taken hourly for five hours.
    Drought stressed plants had not mobilized their carbohydrate reserves from their source
    leaves overnight and thus maintained cell viability and therefore higher chlorophyll
    fluorescence values throughout the incubation with the opposite being true for nonstressed
    plants. Fiber lint yield and fiber properties were measured at the conclusion of
    the 2005 season in College Station and the 2006 season in College Station and Lubbock for comparison with the fluorescence data. Five genotypes, 'Acala 1517-99', 'Deltapine
    491' (PVP no. 200100159), 'Tamcot CAMD-E', 'Tamcot 22' and TAM 89E-51, an
    unreleased breeding line, were selected based on field evaluation results in a preliminary
    study in 2005 to be included in a diallel analysis to determine the heritability of
    fluorescence measurements. Genotype x treatment effects complicated the classification
    of genotypic responses to drought. Few and inconsistent correlations were found among
    fluorescence values and lint yield or fiber properties. The diallel analysis did not
    identify general combining ability or specific combining ability effects for chlorophyll
    fluorescence measurements. Thus this procedure provides little potential in selecting
    plants for drought tolerance when plants are grown under field culture. Selection among
    Tamcot 22 and TAM 89E-51 plants for high and low genotypes according to
    fluorescence values did not yield progeny different from unselected Tamcot 22 and
    TAM 89E-51.

ETD Chair

publication date

  • May 2008