The hot gas temperature in gas turbine engines is far above the permissible metal temperatures. Advanced cooling technologies must be applied to cool the blades, so they can withstand the extreme conditions. Film cooling is widely used in modern high temperature and high pressure blades as an active cooling scheme. In this study, the film cooling effectiveness in different regions of gas turbine blades was investigated with various film hole/slot configurations and mainstream flow conditions. The study consisted of four parts: 1) effect of upstream wake on blade surface film cooling, 2) effect of upstream vortex on platform purge flow cooling, 3) influence of hole shape and angle on leading edge film cooling and 4) slot film cooling on trailing edge. Pressure sensitive paint (PSP) technique was used to get the conduction-free film cooling effectiveness distribution. For the blade surface film cooling, the effectiveness from axial shaped holes and compound angle shaped holes were examined. Results showed that the compound angle shaped holes offer better film effectiveness than the axial shaped holes. The upstream stationary wakes have detrimental effect on film effectiveness in certain wake rod phase positions. For platform purge flow cooling, the stator-rotor gap was simulated by a typical labyrinth-like seal. Delta wings were used to generate vortex and modeled the passage vortex generated by the upstream vanes. Results showed that the upstream vortex reduces the film cooling effectiveness on the platform. For the leading edge film cooling, two film cooling designs, each with four film cooling hole configurations, were investigated. Results showed that the shaped holes provide higher film cooling effectiveness than the cylindrical holes at higher average blowing ratios. In the same range of average blowing ratio, the radial angle holes produce better effectiveness than the compound angle holes. The seven-row design results in much higher effectiveness than the three-row design. For the trailing edge slot cooling, the effect of slot lip thickness on film effectiveness under the two mainstream conditions was investigated. Results showed thinner lips offer higher effectiveness. The film effectiveness on the slots reduces when the incoming mainstream boundary layer thickness decreases.