Molecular basis for plant osmotic stress tolerance
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More than 20 genes in the Arabidopsis genome encode proteins similar to phosphatases that act on the carboxyl-terminal domain (CTD) of RNA polymerase II (Koiwa, 2006). Arabidopsis thaliana mutants containing T-DNA insertions in three isoforms of this gene family, and RNAi silencing of another isoform revealed their importance in plant growth, development and stress responses (Koiwa et al., 2002; Koiwa et al., 2004; Ueda et al., 2008; Aksoy et al., 2013; Fukudome et al., 2014). The current goal of the project is to identify the function of CTD-phosphatase-like 1 (CPL1), in plant stress response, as this gene is unique to plants and contains double stranded RNA binding motives. We will use stress-inducible RD29a-LUC transgene as a model target gene to study the function of CPL1. In this project, we focus on role of CPL1 in splicing-like RNA degradation pathway.