Isolation and characterization of vascular endothelial cells from murine heart and lung. Chapter

abstract

• The formation of blood vessel networks is a fundamental event in vertebrate embryo development. Angiogenesis and vasculogenesis are the essential processes in vascular formation. Endothelial cells play a key role during angiogenesis and vasculogenesis, and cultured vascular endothelial cells provide an indispensable model for exploring the molecular mechanisms of angiogenesis and vasculogenesis. In this chapter, we described a protocol using PECAM-1-coated Dynabeads for the isolation of vascular endothelial cells from mouse heart and lung. This method will provide up to 10(7) endothelial cells with high purity (>85%). The isolated endothelial cells retain their in vivo characteristics, such as the expression of the cell surface markers PECAM-1 and ICAM-2.

authors

• Peng, Xu

author list (cited authors)

• Jin, Y., Liu, Y., Antonyak, M., & Peng, X. u.

citation count

• 16

complete list of authors

• Jin, Yixin||Liu, Yang||Antonyak, Marc||Peng, Xu

editor list (cited editors)

• Peng, X., & Antonyak, M.

Book Title

• CARDIOVASCULAR DEVELOPMENT: METHODS AND PROTOCOLS

publication date

• February 2012

publisher

• Springer Nature  Publisher

published in

• Methods in molecular biology (Clifton, N.J.)  Journal

keywords

• Animals
• Antigens, CD
• Cell Adhesion Molecules
• Cell Movement
• Cell Separation
• Dissection
• Endothelial Cells
• Flow Cytometry
• Gelatin
• Gene Knockout Techniques
• Lung
• Magnets
• Mice
• Mice, Transgenic
• Microspheres
• Myocardium
• Platelet Endothelial Cell Adhesion Molecule-1
• Signal Transduction

PubMed Central ID

• 22222529

Digital Object Identifier (DOI)

• 10.1007/978-1-61779-523-7_14

International Standard Book Number (ISBN) 13

• 978-1-61779-522-0

start page

• 147

end page

• 154

volume

• 843

URL

• http://dx.doi.org/10.1007/978-1-61779-523-7_14