Isolation and characterization of vascular endothelial cells from murine heart and lung. Chapter uri icon

abstract

  • The formation of blood vessel networks is a fundamental event in vertebrate embryo development. Angiogenesis and vasculogenesis are the essential processes in vascular formation. Endothelial cells play a key role during angiogenesis and vasculogenesis, and cultured vascular endothelial cells provide an indispensable model for exploring the molecular mechanisms of angiogenesis and vasculogenesis. In this chapter, we described a protocol using PECAM-1-coated Dynabeads for the isolation of vascular endothelial cells from mouse heart and lung. This method will provide up to 10(7) endothelial cells with high purity (>85%). The isolated endothelial cells retain their in vivo characteristics, such as the expression of the cell surface markers PECAM-1 and ICAM-2.

author list (cited authors)

  • Jin, Y., Liu, Y., Antonyak, M., & Peng, X. u.

editor list (cited editors)

  • Peng, X., & Antonyak, M.

Book Title

  • Methods in Molecular Biology

publication date

  • January 1, 2012 11:11 AM