Absence of bovine leukosis virus in semen of seropositive bulls. Academic Article

abstract

• A polymerase chain reaction (PCR)-based detection system was established to identify the presence of bovine leukosis virus (BLV) DNA in bovine semen. Seventy-nine bulls were included in the study. Serum, peripheral blood leukocytes, and semen were collected from each of the 79 bulls. The BLV-specific antibody was detected in serum by agar gel immunodiffusion and viral DNA in blood and semen by PCR. Serologically, 29 of the 79 bulls were BLV positive. Twenty-seven of the 29 seropositive bulls and 1 of the seronegative bulls had BLV DNA in peripheral blood leukocytes. All 79 bulls tested PCR negative for the presence of BLV in semen. This data is strong evidence that properly collected semen from BLV seropositive bulls will not contribute to dissemination of this viral infection.

authors

• Choi, K. Yeon

published proceedings

• J Vet Diagn Invest

author list (cited authors)

• Choi, K. Y., Monke, D., & Stott, J. L.

citation count

• 14

complete list of authors

• Choi, K Yeon||Monke, Don||Stott, Jeffrey L

publication date

• September 2002

publisher

• SAGE Publications  Publisher

published in

• Journal of Veterinary Diagnostic Investigation  Journal

keywords

• Animals
• Antibodies, Viral
• Blotting, Southern
• Cattle
• DNA, Viral
• Enzootic Bovine Leukosis
• Leukemia Virus, Bovine
• Leukocytes
• Male
• Polymerase Chain Reaction
• Semen

PubMed Central ID

• 12296392

Digital Object Identifier (DOI)

• 10.1177/104063870201400507

start page

• 403

end page

• 406

volume

• 14

issue

• 5

URL

• http://dx.doi.org/10.1177/104063870201400507