Synthesis and assembly of adenovirus type 2 polypeptides. II. Reversible inhibition of hexon assembly at 42 degrees.
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Hexon polypeptides (monomers) synthesized at 42 do not assemble into capsomeres (trimers) until temperature stepdown to 37. Structural studies of monomer and trimer show them to be identical in molecular weight and tryptic peptide patterns. Cyanide and mercaptoethanol, both of which can interact with sulfhydryl groups, inhibit hexon assembly. Therefore, the oxidation state of each sulfhydryl group of cysteine was examined, but was found to be the same for free polypeptides and capsomeres. These observations suggest that factors other than the primary structure of the polypeptides are important for in vivo assembly. 1974.
author list (cited authors)
Leibowitz, J., & Horwitz, M. S.
complete list of authors
Leibowitz, J||Horwitz, MS