Synthesis of virus-specific RNA in permeabilized murine coronavirus-infected cells. Academic Article

abstract

• We have developed a permeabilized cell system for assaying mouse hepatitis virus-specific RNA polymerase activity. This activity was characterized as to its requirements for mono- and divalent cations, requirements for an exogenous energy source, and pH optimum. This system faithfully reflects MHV-specific RNA synthesis in the intact cell, with regard to both its time of appearance during the course of infection and the products synthesized. The system is efficient and the RNA products were identical to those observed in intact MHV-infected cells as judged by agarose gel electrophoresis and hybridization. Permeabilized cells appear to be an ideal system for studying coronavirus RNA synthesis since they closely mimic in vivo conditions while allowing much of the experimental flexibility of truly cell-free systems.

authors

• Leibowitz, Julian

published proceedings

• Virology

author list (cited authors)

• Leibowitz, J. L., & DeVries, J. R.

citation count

• 16

complete list of authors

• Leibowitz, JL||DeVries, JR

publication date

• September 1988

publisher

• Elsevier  Publisher

published in

• Virology  Journal

keywords

• Cell Line
• Cell Membrane Permeability
• Deoxyribonucleases
• Hydrogen-Ion Concentration
• Magnesium
• Murine Hepatitis Virus
• Nucleic Acid Hybridization
• RNA Nucleotidyltransferases
• RNA, Messenger
• RNA, Viral
• Ribonucleases
• Rna-dependent Rna Polymerase
• Time Factors

PubMed Central ID

• 2842958

Digital Object Identifier (DOI)

• 10.1016/0042-6822(88)90147-x

start page

• 66

end page

• 75

volume

• 166

issue

• 1

URL

• http://dx.doi.org/10.1016/0042-6822(88)90147-x